Barcode ID

BOLD enables researchers, students and citizen scientists to submit DNA barcode sequences for identification against a reference library that includes sequences from more than 1.2 million species.

Identification Engine

Database

ANIMAL LIBRARY (PUBLIC)

Non-redundant COI sequences of at least 500bp within the 648bp barcode region.

  • Marker(s): COI-5P
  • Sequences: 8,585,730

ANIMAL:PUBLIC

ANIMAL SPECIES-LEVEL LIBRARY (PUBLIC + PRIVATE)

COI sequences with species designation of at least 500bp within the 648bp barcode region.

  • Marker(s): COI-5P
  • Sequences: 9,480,417

ANIMAL:SPECIES

ANIMAL LIBRARY (PUBLIC+PRIVATE)

Non-redundant COI sequences of at least 500bp within the 648bp barcode region.

  • Marker(s): COI-5P
  • Sequences: 9,992,381

ANIMAL:PUBLIC+PRIVATE

VALIDATED CANADIAN ARTHROPOD LIBRARY

A validated set of Canadian arthropod records, produced by Pentinsaari et al. (in prep.).

  • Marker(s): COI-5P
  • Sequences: 100,374

VALIDATED:CANREF22

PLANT LIBRARY (PUBLIC)

Non-redundant plant rbcL, matK, and ITS sequences of at least 200bp in length.

  • Marker(s): matK, rbcL, ITS1, ITS2, ITS
  • Sequences: 455,607

PLANT:PUBLIC

FUNGI LIBRARY (PUBLIC)

Non-redundant fungal ITS and 18S sequences of at least 200bp in length.

  • Marker(s): 18S, ITS1, ITS2, ITS
  • Sequences: 329,133

FUNGI:PUBLIC

ANIMAL SECONDARY MARKERS (PUBLIC)

Non-redundant 18S and 12S sequences of at least 200bp in length.

  • Marker(s): 18S, 12S, CAD, ITS, ITS1, ITS2
  • Sequences: 67,500

ANIMAL:PUBLIC-SECONDARY

VALIDATED ANIMAL RED LIST LIBRARY

A validated dataset of animal Red List species, produced by Monckton et al. (in prep.).

  • Marker(s): COI-5P
  • Sequences: 69,506

VALIDATED:IUCN

Operating Mode

Rapid Species Search

Ideal when only looking for species or BIN match.

  • Search Depth: 94% similarity
  • Max Hits: 25 per sequence
  • Min Overlap: 100
  • Max Submission: 1000 sequences

AVG. ANALYSIS TIME: 1s/sequence

Genus and Species Search

Ideal when only looking looking for species or BIN match.

  • Search Depth: 90% similarity
  • Max Hits: 50 per sequence
  • Min Overlap: 100
  • Max Submission: 200 sequences

AVG. ANALYSIS TIME: 4s/sequence

Exhaustive Search

Required for matches to subfamily and above.

  • Search Depth: 75% similarity
  • Max Hits: 100 per sequence
  • Min Overlap: 100
  • Max Submission: 100 sequences

AVG. ANALYSIS TIME: 10s/sequence

The BOLD Identification (ID) Engine is intended for data exploration and analysis. To ensure responsible use, outputs that contain private data may not be reproduced, modified, or published without the private data owner’s explicit written consent. Inquiries regarding proposals for collaborative research or permission to use private data should be directed to support@boldsystems.org. By using this tool, users agree to respect these guidelines.