BOLD enables researchers, students and citizen scientists to submit DNA barcode
sequences
for identification against a reference library that includes sequences from more than
1.2
million species.
Identification Engine
Due to scheduled electrical maintenance by the
University of Guelph, BOLD Systems will have reduced analysis capacity from Apr 25, 2025 12:00 pm
until Apr 28, 2025 10:30 am EDT.
Database
ANIMAL LIBRARY (PUBLIC)
Non-redundant COI sequences of at least 500bp within the 648bp barcode region.
Marker(s): COI-5P
Sequences: 8,492,012
ANIMAL:PUBLIC
ANIMAL SPECIES-LEVEL LIBRARY (PUBLIC + PRIVATE)
COI sequences with species designation of at least 500bp within the 648bp barcode region.
Marker(s): COI-5P
Sequences: 9,317,452
ANIMAL:SPECIES
ANIMAL LIBRARY (PUBLIC+PRIVATE)
Non-redundant COI sequences of at least 500bp within the 648bp barcode region.
Marker(s): COI-5P
Sequences: 9,940,653
ANIMAL:PUBLIC+PRIVATE
VALIDATED CANADIAN ARTHROPOD LIBRARY
A validated set of Canadian arthropod records, produced by Pentinsaari et al. (in prep.).
Marker(s): COI-5P
Sequences: 100,379
VALIDATED:CANREF22
PLANT LIBRARY (PUBLIC)
Non-redundant plant rbcL, matK, and ITS sequences of at least 200bp in length.
Marker(s): matK, rbcL, ITS1, ITS2, ITS
Sequences: 455,449
PLANT:PUBLIC
FUNGI LIBRARY (PUBLIC)
Non-redundant fungal ITS and 18S sequences of at least 200bp in length.
Marker(s): 18S, ITS1, ITS2, ITS
Sequences: 327,762
FUNGI:PUBLIC
ANIMAL SECONDARY MARKERS (PUBLIC)
Non-redundant 18S and 12S sequences of at least 200bp in length.
Marker(s): 18S, 12S, CAD, ITS, ITS1, ITS2
Sequences: 66,808
ANIMAL:PUBLIC-SECONDARY
VALIDATED ANIMAL RED LIST LIBRARY
A validated dataset of animal Red List species, produced by Monckton et al. (in prep.).
Marker(s): COI-5P
Sequences: 69,509
VALIDATED:IUCN
Operating Mode
Rapid Species Search
Ideal when only looking for species or BIN match.
Search Depth: 94% similarity
Max Hits: 25 per sequence
Min Overlap: 100
Max Submission: 1000 sequences
AVG. ANALYSIS TIME: 1s/sequence
Genus and Species Search
Ideal when only looking looking for species or BIN match.