Barcode ID

BOLD enables researchers, students and citizen scientists to submit DNA barcode sequences for identification against a reference library that includes sequences from more than 1.2 million species.

Identification Engine

Database

ANIMAL LIBRARY (PUBLIC)

Non-redundant COI sequences of at least 500bp within the 648bp barcode region.

  • Marker(s): COI-5P
  • Sequences: 8,293,790

ANIMAL:PUBLIC

ANIMAL SPECIES-LEVEL LIBRARY (PUBLIC + PRIVATE)

COI sequences with species designation of at least 500bp within the 648bp barcode region.

  • Marker(s): COI-5P
  • Sequences: 8,989,361

ANIMAL:SPECIES

ANIMAL LIBRARY (PUBLIC+PRIVATE)

Non-redundant COI sequences of at least 500bp within the 648bp barcode region.

  • Marker(s): COI-5P
  • Sequences: 9,785,148

ANIMAL:PUBLIC+PRIVATE

VALIDATED CANADIAN ARTHROPOD LIBRARY

A validated set of Canadian arthropod records, produced by Pentinsaari et al. (in prep.).

  • Marker(s): COI-5P
  • Sequences: 100,381

VALIDATED:CANREF22

PLANT LIBRARY (PUBLIC)

Non-redundant plant rbcL, matK, and ITS sequences of at least 200bp in length.

  • Marker(s): matK, rbcL, ITS1, ITS2, ITS
  • Sequences: 454,835

PLANT:PUBLIC

FUNGI LIBRARY (PUBLIC)

Non-redundant fungal ITS and 18S sequences of at least 200bp in length.

  • Marker(s): 18S, ITS1, ITS2, ITS
  • Sequences: 322,953

FUNGI:PUBLIC

ANIMAL SECONDARY MARKERS (PUBLIC)

Non-redundant 18S and 12S sequences of at least 200bp in length.

  • Marker(s): 18S, 12S, CAD, ITS, ITS1, ITS2
  • Sequences: 65,340

ANIMAL:PUBLIC-SECONDARY

VALIDATED ANIMAL RED LIST LIBRARY

A validated dataset of animal Red List species, produced by Monckton et al. (in prep.).

  • Marker(s): COI-5P
  • Sequences: 69,509

VALIDATED:IUCN

Operating Mode

Rapid Species Search

Ideal when only looking for species or BIN match.

  • Search Depth: 94% similarity
  • Max Hits: 25 per sequence
  • Min Overlap: 100
  • Max Submission: 1000 sequences

AVG. ANALYSIS TIME: 1s/sequence

Genus and Species Search

Ideal when only looking looking for species or BIN match.

  • Search Depth: 90% similarity
  • Max Hits: 50 per sequence
  • Min Overlap: 100
  • Max Submission: 200 sequences

AVG. ANALYSIS TIME: 4s/sequence

Exhaustive Search

Required for matches to subfamily and above.

  • Search Depth: 75% similarity
  • Max Hits: 100 per sequence
  • Min Overlap: 100
  • Max Submission: 100 sequences

AVG. ANALYSIS TIME: 10s/sequence